A Comprehensive Guide to Growing Mushrooms from ‘Spores’
Mushroom Spores


What are mushroom spores?

Mushrooms are not only a delicious addition to culinary dishes but also offer numerous health benefits to humans. 

To start off with, we need to know what ‘mushroom spores’ are. 

Mushroom spores are the microscopic reproductive units of fungi, and are similar to seeds in plants – as they contain the genetic material necessary for the growth and development of a new fungal organism. Spores are produced in the gills, pores, and other specialized structures on the underside of the mushroom’s cap. 

When ‘mature mushrooms’ release their spores, they are dispersed into the surrounding environment. Spores can be carried by air currents or water – allowing them to potentially colonize new areas and start the growth of new mushrooms. 

Spores vary in size, shape, color, and even texture – depending on the mushroom species. They are often produced in large quantities, but only a small fraction will successfully germinate and develop into new mycelium (the vegetative part of the fungus). These spores are also highly resistant and can survive adverse conditions until they find a suitable environment for germination. 

Cultivating Mushrooms from Spores:

To cultivate mushrooms from spores, they need to be provided with the right conditions and nutrients to germinate and develop into mycelium. The mycelium then colonizes a growing medium or substrate – eventually forming the familiar fruiting bodies we recognize as ‘mushrooms’. 

Even though growing mushrooms from spores can be a more advanced and time-consuming process compared to using pre-existing mushroom spawn or mycelium, you can ensure successful cultivation with the sterile techniques shared in this blog. Let’s explore the step-by-step process of growing mushrooms from spores – specifically in the beautiful province of British Columbia, Canada. 

Mushroom Spores

Understanding Basic Concepts to Grow Mushrooms from Spores

Before we dive into a more detailed procedure, understanding a few basic concepts and gathering the necessary supplies is important. 

Optimal Conditions to Germinate

Mushrooms are the fruiting bodies of fungi, and they reproduce through spores (which are similar to seeds in plants). Spores are tiny, reproductive units that contain the genetic material required to develop into a full-grown mushroom. To successfully cultivate mushrooms, we have to provide them with the optimal conditions for the spores to germinate and develop into mycelium. 

Required Supplies:

Required supplies for growing mushrooms from spores include mushroom spores (or spawn), growing medium, sterilized containers, spawn or substrate inoculation tools, temperature and humidity control, lighting, and mist or spray bottles. 

  1. Mushroom spores or spawns:

Choose a mushroom species suitable for cultivation in the region you’re in. For example, in British Columbia, popular choices include oyster mushrooms (Pleurotus spp.), shiitake mushrooms (Lentinula edodes), and lion’s mane mushrooms (Hericium Erinaceus). To obtain spores or spawn, you can visit local suppliers or online sources. 

  1. Growing medium:

Common options for your growing medium include straw, sawdust, wood chips, or even compost. Use a substrate that is appropriate for the mushroom species that you intend to grow. 

  1. Sterilized containers:

To use as sterilized containers, plastic bags or buckets can come in handy. 

  1. Spawn or substrate inoculation tools:

These tools include gloves, a mask, and a clean working environment to minimize contamination while growing your species. 

  1. Temperature and humidity control:

Consider using a thermometer, hygrometer, and a humidifier (or mister) to maintain optimal conditions for mushroom growth. 

  1. Lighting:

Mushrooms don’t require direct sunlight, but they still need a source of indirect light or darkness – depending on the species. 

  1. Mist or spray bottle:

This is useful for maintaining moisture levels during the growth process.

  1. Additional tools for help:

A pressure cooker or autoclave for sterilization is also important. A clean working surface is ideal, and a spray bottle for misting from time to time is also useful. 

A Step-by-Step Guide to Growing Mushrooms from Spores:

Step by Step Guide for Mushroom Spores

Step 1: Choose a mushroom species

Research and select a mushroom species suitable for cultivation in your area. Ensure that the chosen species align with the climate and growing conditions in your area. 

Step 2: Prepare the growing substrate

Different mushrooms require different growing substrates – for example, oyster mushrooms thrive on a mix of straw and coffee grounds, while shiitake mushrooms prefer a blend of hardwood sawdust and wheat bran. Follow specific instructions for preparing the substrate as recommended by your supplier or a mycologist (mushroom expert). 

Step 3: Sterilize the Substrate 

To prevent contamination from competing organisms, sterilize your substrate using a pressure cooker or autoclave. Follow the manufacturer’s guidelines for sterilization time and temperature, and let the substrate cool before proceeding. 

Step 4: Inoculate the Substrate

Once the substrate has cooled down, mix in the mushroom spawn or spores. Distribute the spawn evenly throughout the substrate to ensure uniform colonization, and wear gloves (and a mask) to maintain a sterile environment during the process. 

Step 5: Containerize the Substrate

Transfer the inoculated substrate to a clean container – such as a plastic bag or bucket. Poke small holes in the container for proper air circulation. 

Step 6: Incubation Period

Place the container in a warm and dark location with an optimal temperature and humidity for the specific mushroom species. Maintain the appropriate conditions by monitoring temperature and humidity levels. 

Step 7: Monitor and Mist

For best results, regularly check the substrate for moisture levels. If it appears dry, mist it a little bit with water using a spray bottle. Ensure that the substrate remains moist, but not soaking wet. This step is crucial for the mycelium to develop and spread throughout the substrate. 

Step 8: Provide Indirect Sunlight

Once the mycelium has started to colonize the substrate, introduce indirect sunlight to stimulate the formation of mushrooms. By avoiding direct sunlight, you protect mushroom growth – as too much sunlight can be detrimental. Some species (such as oyster mushrooms) require a period of darkness to trigger fruiting. Follow the specific ‘light requirements’ for the mushroom species that you are growing. 

Step 9: Adjust Temperature and Humidity for Fruiting

As your mycelium continues to grow and develop, adjust the temperature and humidity to encourage fruiting (this part gets a little technical and you may need more help and guidance). Different mushroom species have varying temperature requirements, but a range of 18-24°C (64-75°F) is suitable for many common mushrooms. For best results, consult with a mycologist and use a thermometer/hygrometer to monitor and maintain optimal conditions. 

Step 10: Harvesting

Mushrooms start to appear as small pinheads, gradually growing in size. You should harvest the mushrooms at their prime stage when the caps are fully developed (but still firm) and before the gills start to release spores. Using clean scissors or a sharp knife, cut the mushrooms at the ‘base’ of the stem. 

Step 11: Second or Third Flush

After the initial harvest, some mushroom species are capable of producing a second or even third flush of mushrooms. To encourage additional fruiting, follow the same steps as before – ensuring proper temperature, humidity, and lighting conditions. A word of caution: subsequent flushes may require longer periods of time to develop, compared to the initial flush. 

Step 12: Troubleshooting or Preventing Contamination

Throughout the growing process, it is important to maintain a clean and sterile environment to prevent contamination by molds or bacteria. 

For this, we give you a few tips:

  • Always wear gloves and a mask when handling the substrate, spawn, or mushrooms.
  • Keep your workspace clean and sanitized.
  • Sterilize all equipment before and after use.
  • Use quality spores or spawn from reputable places.
  • Properly pasteurize or sterilize the substrate to eliminate bacteria.
  • If you encounter contamination, it is best to discard the affected substrate to prevent further spread.

The Bottom Line

Growing mushrooms straight from the spores can be a fascinating and fulfilling experience, but if you follow the steps outlined in this guide. By providing the necessary care, you can cultivate your own mushrooms right in the comfort of your home. Remember to choose suitable mushroom species for your region – providing them with optimal conditions for mycelium growth. Maintain the proper temperature, humidity, and lighting throughout the process. 

With patience and practice, you can enjoy a harvest of delicious, nutritious, and sacred mushrooms – adding a touch of nature’s goodness to your culinary and other therapeutic endeavors. 

About D&N Sporeworks

Based in the heart of British Columbia, D&N Sporeworks is a dedicated family-run business that takes pride in its passion for the hobby of exploring spore genetics. With a strong commitment to maintaining a sterile environment, we ensure that all our work is conducted with utmost care and precision.

At D&N Sporeworks, our goal is to offer the finest genetics to our valued customers. We boast an extensive collection of diverse strains, each carefully selected for its quality and unique characteristics. In our ongoing effort to enhance your experience, we consistently introduce new strains on a monthly basis. We encourage you to visit our website regularly to stay updated on our ever-expanding selection.

Our commitment to providing excellent customer service extends seven days a week, and we strive to process orders within a prompt timeframe of 1-3 business days.

Choose D&N Sporeworks for an unparalleled experience in exploring spore genetics. Trust in our expertise, sterile practices, and dedication to customer satisfaction. Begin your journey with us today and discover the world of possibilities that await.


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